The InSight-X3 ultrafast tunable laser is based on patented technology featuring a broad 680 nm to 1300 nm continuous, gap free, tuning curve from a single beam with nearly double the tuning range of legacy Ti:Sapphire lasers. The InSight is designed with the peak of the tuning curve centered in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry. The InSight X3+ additionally provides the highest powers at the longest wavelengths of 1280–1300 nm for excitation of far red fluorophores such as Alexa-680.